Basic and clinical immunology – 3033: Structural analyses and allergencity predicition of sapodilla (Manikara zapota) acidic thaumatin-like protein by bioinformatics

نویسندگان

  • YP Venkatesh
  • HG Ashok Kumar
چکیده

Background Oral allergy syndrome to sapodilla plum is caused by a 21 kD allergen existing as two isoforms: an acidic thaumatin-like protein (TLP-1) and a basic thaumatin-like protein (TLP-2). The composite sequence of TLP-1 comprising the N-terminal sequence 1-7 (A-T-F-D-V/I-V-N-) and the deduced sequence 8-207 (GenBank JN624813.1) was used for this analysis. The major objective of this study was to analyze the structural features and allergenicity prediction of TLP-1 by bioinformatics.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Food allergy and anaphylaxis – 2046. Cloning and characterization of the gene for acidic thaumatin-like protein, an important allergen from sapodilla plum (manilkara zapota)

Methods A case of oral allergy syndrome to sapodilla and custard apple was investigated following approval by Institutional Ethics Committee. Sapodilla allergy was confirmed by diagnostic tests (SPT and allergen-specific IgE). Sapodilla proteins were separated on SP-Sepharose by adsorption at pH 4 followed by step elution at pH 5 (SP1), and with increasing NaCl – 0.1 M (SP2) and 0.2 M (SP3). Fo...

متن کامل

Pharmacological and computational evaluation of Sapodilla and its constituents for therapeutic potential in hyperactive gastrointestinal disorders

Objective(s): This study was designed to investigate various gastrointestinal effects of Manilkara zapota (Sapodilla), exploring its anti-diarrheal, anti-secretary, anti-spasmodic, anti-ulcer and anti-motility potential.Materials and Methods: Antidiarrheal and anti-secretary activities were investigated using castor oil induced diarrhea ...

متن کامل

Molecular Characterization of the Epstein-Barr Virus BGLF2 Gene, its Expression, and Subcellular Localization

Background: Epstein–Barr virus (EBV) is a universal herpes virus which can cause a life-long and largely asymptomatic infection in the human population. However, the exact pathogenesis of the EBV infection is not well known.Objective: A comprehensive bioinformatics prediction was carried out for investigating the molecular properties of the BGLF2 and to a...

متن کامل

Heterologous Expression of the Secale cereal Thaumatin-Like Protein in Transgenic Canola Plants Enhances Resistance to Stem Rot Disease

Canola (Brassica napus L.) is an important oilseed crop. A serious problem in cultivation of this crop andyield loss, are due to fungal disease stem rot caused by Sclerotinia sclerotiorum. The pathogenesis-related(PR) proteins have the potential for enhancing resistance against fungal pathogen. Thaumatin-like proteins(TLPs) have been shown to have antifungal activity on variou...

متن کامل

Bioinformatics analysis and expression of a truncated form of Proteus mirabilis Pta protein as a novel vaccine target against urinary tract infection

Introduction: Pathogenic strains of Proteus mirabilis have important roles in urinary tract infection. Proteus toxic agglutinin (Pta) is amongst the most important virulence factors of P. mirabilis. This protein has a conserved sequence present in all the strains which could be evaluated as a novel vaccine target against them. The aims of the current study were the expression, purification and ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2013